KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Aβ Antibody HJ3.4 | (Bero et al., 2012) | N/A |
| Mouse monoclonal anti-Aβ Antibody HJ5.1 | (Bero et al., 2011) | N/A |
| Mouse monoclonal anti-Aβ1–40 Antibody HJ2 | (Bero et al., 2011) | N/A |
| Mouse monoclonal anti-Aβ1–42 Antibody HJ7.4 | (Bero et al., 2011) | N/A |
| Mouse monoclonal anti-apoE Antibody (HJ15.6) | (Liao et al., 2015) | N/A |
| Mouse monoclonal anti-apoE Antibody (HJ15.7) | (Liao et al., 2015) | N/A |
| Mouse monoclonal anti-apoE Antibody (HJ15.4) | (Liao et al., 2015) | N/A |
| Mouse monoclonal anti-Aβ (82E1) Antibody | IBL-America | IBL-America Cat# 10323; RRID:AB_10707424 |
| Rat anti mouse CD45 Antibody | Bio-Rad/AbD Serotec | Cat# MCA1388 RRID:AB_321729 |
| Goat anti-Rat IgG Secondary Antibody, Biotin conjugate | Thermo Fisher Scientific | Cat# PA1-84402 RRID:AB_931503 |
| Rat monoclonal anti-LAMP1 Antibody | Iowa Developmental Studies Hybridoma bank | DSHB # 1D4B |
| Goat polyclonal anti-iba1 Antibody | Abcam | Abcam # ab5076 |
| GFAP Monoclonal Antibody (GA5), Alexa Fluor 488, eBioscience(TM), Thermo Fisher Scientific | Thermo Fisher Scientific | Cat# 53-9892-82 also 53-9892 RRID:AB_10598515 |
| Rabbit polyclonal anti-pan-ASO Antibody | Ionis Pharmaceuticals | # 13545 |
| Donkey Anti-Mouse Donkey anti-mouse IgG-HRP Polyclonal, HRP Conjugated antibody | Santa Cruz Biotechnology | Cat# sc-2096 RRID:AB_641168 |
| β-Tubulin (H-235) antibody | Santa Cruz Biotechnology | Cat# sc-9104 RRID:AB_2241191 |
| Peroxidase-AffiniPure Goat Anti-Mouse IgG (H + L) antibody | Jackson ImmunoResearch Labs | Cat# 115-035-003 RRID:AB_10015289 |
| Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) antibody | Jackson ImmunoResearch Labs | Cat# 111-035-003 RRID:AB_2313567 |
| Rabbit Anti-Actin Antibody, Unconjugated | Sigma-Aldrich | Cat# A2066 RRID:AB_476693 |
| Purified anti-β-Amyloid1–16 antibody, | BioLegend | Cat# 803001 RRID:AB_2564653 |
| Mouse Anti-Human ApoE Monoclonal, Alexa Fluor 647 Conjugated, Clone WUE-4 antibody | Novus Biologicals | Cat# NB110-60531R RRID:AB_1850315 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11006 also A11006 RRID:AB_2534074 |
| Streptavidin, Alexa Fluor® 568 conjugate antibody | Thermo Fisher Scientific | Cat# S-11226 RRID:AB_2315774 |
| Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 568 Conjugated | Molecular Probes | Cat# A-11057, RRID:AB_142581 |
| Donkey Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 647 Conjugated, | Molecular Probes | Cat# A-31573 also A31573 RRID:AB_2536183 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Trizol | Life Technologies | 15596026 |
| Ethyl Alcohol | Pharmco-Aaper | 11100020S |
| Sucrose | Sigma-Aldrich | S0389 |
| Albumin, Bovine Fraction V (BSA) | RPI Research Products International | 9048-46-86 |
| X-34 dye | Gift from Dr. Robert Mach (Styren et al., 2000) | N/A |
| Critical Commercial Assays | ||
| Lumigen ECL Ultra (TMa-6) | Thermo Fisher Scientific | # NC024069 |
| Micro BCA Protein Assay Kit | Thermo Fisher Scientific | # 23235 |
| TaqMan™ Universal PCR Master Mix | Thermo Fisher Scientific | # 4304437 |
| High-Capacity RNA-to-cDNA™ Kit | Thermo Fisher Scientific | # 4387406 |
| RNeasy Mini Kit (50) | Quiagen | # 74104 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| B6.129P2-Apoetm3(APOE*4)Mae N8 | Taconic | 1549 |
| B6.129P2-Apoetm2(APOE*3)Mae N8 | Taconic | 1548 |
| B6-Tg(Thy1-APPswe; Thy1-PS1 L166P) | Gift from Dr. Mathias Jucker (Radde et al., 2006) | MGI:3765351 |
| Oligonucleotides | ||
| Anti-apoE ASO sequence: 5′-GGTGAATCTTTATTAAAC-3′ | Ionis Pharmaceuticals, Inc. | N/A |
| Control ASO sequence: 5′-CCTATAGGACTATCCAGGAA-3′ | Ionis Pharmaceuticals, Inc. | N/A |
| Taqman probe for apoE | Applied Biosystems | Hs00171168_m1 |
| Taqman probe for GAPDH | Applied Biosystems | Mm99999915_g1 Gapdh |
| Recombinant DNA | ||
| Software and Algorithms | ||
| Prism | Graphpad Software | Version 6.01; http://www.graphpad.com |
| Excel | Microsoft | https://products.office.com/en/excel |
| Imaris 8 & 9 | Bitplane | http://www.bitplane.com/software/imaris |
| ImageJ | NIH | Version 1.48q/1.50 g; https://imagej.nih.gov/ij/ |
| Other | ||