Figure 4. BMMs gain AC-clearance programming in tissue environments.
(A) Flow cytometric analyses of uninjected CD45.1+ WT BMMs (left) or BMMs transferred into the peritoneum for three weeks (right). (B) Flow cytometric analyses of uninjected CD45.1+ tdTomato+ cells (left) or BMMs transferred intranasally (IN) and analyzed after 39d (right). (C) TNF production by BMMs transferred as described in (A), harvested, and stimulated with TLR ligands. (D) TNF production by BMMs transferred as described in (B), harvested, and stimulated with TLR ligands. SiglecF+ cells are gated as shown in panel B. All data are representative of three independent experiments. See also Figure S4.