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. 2017 Dec 4;6:e28415. doi: 10.7554/eLife.28415

Figure 4. Trailblazers are a small subpopulation of cells with a distinct expression signature that exist at the invasive front of the neural crest stream, regardless of developmental stage.

Figure 4.

(A) Heatmap with hierarchical clustering of all single cells from the invasive front at HHSt11, 13 and 15 (n = 187 cells), based upon differentially expressed genes from all invasive front comparisons and invasive front compares to stream at HHSt13 and HHSt15 (n = 760 genes). (B) Pearson correlation matrix of the average expression profile of all subpopulations based upon differentially expressed genes from all invasive front comparisons and comparisons between clusters and all other in vivo cells (n = 3555 genes). (C) PCA of all single cells from the invasive front at all three developmental time points, based upon differentially expressed genes from all invasive front comparisons and invasive front compares to stream at HHSt13 and HHSt15 (n = 760 genes).

Figure 4—source data 1. Single-cell RNA-seq differential expression gene lists for all invasive front subpopulation comparisons.
All differential expression thresholds were set at log2 FC greater than 2 or less than −2 and adjusted p value less than 0.05. Individual comparisons of invasive front subpopulations to all other cells isolated in vivo are displayed as individual tabs within the spreadsheet. Mean RPKM and the percentage of cells within each subpopulation that express each gene are also listed.
elife-28415-fig4-data1.xlsx (342.6KB, xlsx)
DOI: 10.7554/eLife.28415.013