Video 1. RNAscope fluorescence signal of Tescalcin expression.

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DOI: 10.7554/eLife.28415.021

A typical 3D confocal image z-stack of a HHSt13 chick embryo highlighting the neural crest cell migratory stream adjacent to rhombomere four with cells labeled for Tescalcin by RNAscope and neural crest cells marked by HNK1 immunohistochemistry. The HNK1 membrane label was used to mark the boundaries of neural crest cells and mask the Tescalcin channel in 3D. The locations of RNAscope label for Tescalcin were detected and assigned to individual cells using the HNK1 boundaries. The cells were then colored by the number of detected Tescalcin RNAscope label spots with red being the highest and blue the lowest. A typical cell is approximately 20–30 um in diameter.