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. 2017 Nov 30;12:8573–8585. doi: 10.2147/IJN.S139126

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© 2017 Bahadoran et al. This work is published and licensed by Dove Medical Press Limited

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Analysis of the inserted gene (H5-GFP) by using restriction enzyme digestion and polymerase chain reaction.

Notes: M: GeneRulerTM DNA ladder Kit, 1 Kbp (Fermentas, Burlington, Canada); lane 1, 2, 3: single digestion with XhoI; lane 4, 5: double digestion with NotI and XhoI. Single digestion of pBud-H5-GFP with XhoI produced a single fragment of about 7,672 bp, which correlate with the expected size of the recombinant DNA plasmid. Double digestion of the plasmid with NotI and XhoI, resulted in two fragments, 4,546 and 3,126 bp related to pBud CE4.1 and the H5-GFP gene, respectively.

Abbreviations: DNA, deoxyribonucleic acid; GFP, green fluorescent protein.