Table 3.
Serum IL-1β, IL-6, and TNF-α levels in mice immunized with PAMAM/pBud, pBud-H5-GFP, PAMAM/pBud-H5-GFP, TAT-PAMAM/pBud-H5-GFP, and TAT-PAMAM/pBud-H5-GFP-IRF3
| Cytokine | Groups | Level of cytokines (pg/mL) at different days post immunization
|
||
|---|---|---|---|---|
| Day 14 | Day 28 | Day 35 | ||
| IL-1β | Control | 18.91±0.48 | 19.04±0.52 | 18.02±0.42 |
| PAMAM/pBud | 17.89±0.52 | 17.1±0.25 | 18.11±0.62 | |
| pBud-H5-GFP | 17.53±0.89 | 16.98±0.52 | 17.88±0.63 | |
| PAMAM/pBud-H5-GFP | 18.01±0.77 | 22.06±0.74 | 28.27±0.95 | |
| TAT-PAMAM/pBud-H5-GFP | 25.5±0.98 | 5.66±1.25 | 22.06±0.84 | |
| TAT-PAMAM/pBud-H5-GFP-IRF3 | 28.73±0.98 | 4.5±1.25 | 23.81±2.32 | |
| IL-6 | Control | 33.39±0.42 | 34.52±0.45 | 38.85±0.45 |
| PAMAM/pBud | 39.33±0.21 | 34.61±0.52 | 33.99±0.45 | |
| pBud-H5-GFP | 40.68±0.54 | 44.39±0.52 | 45.87±0.61 | |
| PAMAM/pBud-H5-GFP | 50.21±0.52 | 40.68±0.68 | 86.2±0.75 | |
| TAT-PAMAM/pBud-H5-GFP | 102.23±0.87* | 102.18±0.44 | 133.11±0.87 | |
| TAT-PAMAM/pBud-H5-GFP-IRF3 | 105.74±0.98* | 70.4±0.58 | 423±0.98* | |
| TNF-α | Control | 9.67±0.42 | 10.11±0.45 | 10.25±0.45 |
| PAMAM/pBud | 5.29±0.36 | 5.88±0.54 | 6.03±0.45 | |
| pBud-H5-GFP | 6.23±0.45 | 6.41±0.35 | 9.67±0.45 | |
| PAMAM/pBud-H5-GFP | 10.42±0.45 | 10.48±0.52 | 139.64±0.74* | |
| TAT-PAMAM/pBud-H5-GFP | 15.03±0.58 | 14.74±0.92 | 203.23±0.87* | |
| TAT-PAMAM/pBud-H5-GFP-IRF3 | 15.34±0.68 | 16.23±0.68 | 271.52±0.75* | |
Note:
*
P<0.05.
Abbreviations: GFP, green fluorescent protein; IL, interleukin; IRF, interferon-regulatory factor; PAMAM, polyamidoamine; TAT, transactivator of transcription; TNF, tumor necrosis factor.