Table 4.

Serum IFN-γ, IL-2, IL-12, and IL-15 levels in mice immunized with PAMAM/pBud, pBud-H5-GFP, PAMAM/pBud-H5-GFP, TAT-conjugated PAMAM/pBud-H5-GFP, and TAT-PAMAM/pBud-H5-GFP-IRF3

Cytokine	Groups	Level of cytokines (pg/mL) at different days post immunization
		Day 14	Day 28	Day 35
INF-γ	Control	12.44±0.42	12.52±0.45	8.34±0.45
	PAMAM/pBud	12.31±0.51	11.9±0.52	12.17±0.45
	pBud-H5-GFP	12.84±0.41	13.89±0.87	13.2±0.62
	PAMAM/pBud-H5-GFP	12.44±0.52	11.59±0.92	24.38±0.72*
	TAT-PAMAM/pBud-H5-GFP	12.48±0.41	12.31±0.82	52.96±0.84*
	TAT-PAMAM/pBUD-H5-GFP-IRF3	37.73±0.74*	71.21±0.98*	126.82±0.84*
IL-2	Control	13.19±0.52	12.81±0.52	11.1±0.45
	PAMAM/pBud	16.9±0.72	11.7±0.82	13.46±0.65
	pBud-H5-GFP	12.78±0.54	14.27±0.82	14.23±0.45
	PAMAM/pBud-H5-GFP	12.83±0.51	32.6±0.92	31.54±0.81*
	TAT-PAMAM/pBud-H5-GFP	14.66±0.65	14.71±0.66	51.71±0.78*
	TAT-PAMAM/pBUD-H5-GFP-IRF3	17.69±0.65	26.95±0.98	153.05±1.25*
IL-12	Control	96±0.45	94.3±0.65	79.2±0.52
	PAMAM/pBud	85±0.45	88±0.45	85±0.45
	pBud-H5-GFP	103.03±0.95	90.44±0.45	99.56±0.45
	PAMAM/pBud-H5-GFP	115±0.92	90.63±0.96	162.09±0.65*
	TAT-PAMAM/pBud-H5-GFP	120.99±0.58	175.84±0.89	253.23±0.85*
	TAT-PAMAM/pBUD-H5-GFP-IRF3	139.64±0.82	162±0.85	580±6.21*
IL-15	Control	26.33±0.52	27.44±0.65	26.89±0.54
	PAMAM/pBud	12.85±0.48	13.52±0.52	14.25±0.42
	pBud-H5-GFP	9.07±1.25	2.44±0.96	11.84±0.52
	PAMAM/pBud-H5-GFP	40.73±0.98	39.01±2.54	72.67±1.25*
	TAT-PAMAM/pBud-H5-GFP	52.96±0.96	40.73±2.25	65.08±3.25*
	TAT-PAMAM/pBUD-H5-GFP-IRF3	61.86±1.25	69.05±4.25	163.64±1.58*

Note:

^*P<0.05.

Abbreviations: GFP, green fluorescent protein; IFN, interferon; IL, interleukin; IRF, interferon-regulatory factor; PAMAM, polyamidoamine; TAT, transactivator of transcription.