TABLE 1 .
Published and custom-designed primers used for in silico and experimental testing
| Primer | Direction | Primer sequence (5′ to 3′) | Reference(s) |
|---|---|---|---|
| Published | |||
| ITS1 (L) | Forward | TCCGTAGGTGAACCTGCGG | 11 |
| ITS1-F (L) | Forward | CTTGGTCATTTAGAGGAAGTAA | 20–23 |
| ITS5 (L) | Forward | GGAAGTAAAAGTCGTAACAAGG | 11 |
| ITS2 (L)a | Reverse | GCTGCGTTCTTCATCGATGC | 11, 21, 23, 28–35 |
| Custom designed | |||
| ITS1-27F (N) | Forward | TACGTCCCTGCCCTTTGTAC | |
| ITS1-30F (N) | Forward | GTCCCTGCCCTTTGTACACA | |
| ITS1-34F (N) | Forward | CTGCCCTTTGTACACACCGC | |
| ITS1-48F (N) | Forward | ACACACCGCCCGTCGCTACT | |
| ITS1-217R (N) | Reverse | TTTCGCTGCGTTCTTCATCG |
a
This published reverse primer was also tested against the four selected custom-designed forward primers. “(L)” designates primers from the literature, and "(N)" indicates primers newly designed for this study.