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. 2017 Dec 13;2(6):e00492-17. doi: 10.1128/mSphere.00492-17

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Copyright © 2017 Hines et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Lipidomics analysis by HILIC-IM-MS. (A) 2D plot of IM drift time versus m/z shows that singly charged (z = 1) lipids occupy a region distinct from that of doubly charge (z = 2) lipids such as CLs. (B) The TIC (0 to 9 min of a 12-min run) from the negative-mode analysis of a lipid mixture suffers from high baseline noise. (C) Extraction of the regions containing z = 1 and z = 2 lipids (dashed outlines) generated an IM-XIC containing only the lipid signals from the mixture of DGs, MGDGs, DGDGs, PGs, CLs, PAs, and lysyl-PGs.