FIG 3 .

Results of analyses of daptomycin-susceptible (N315) and daptomycin-resistant (N315-D8) S. aureus by HILIC-IM-MS. Negative-mode IM-XICs of N315 (raw intensity [Int.] of 101-mg dry pellet shown) (A) and N315-D8 (raw intensity of 77-mg dry pellet shown) (B) reveal that PGs and lysyl-PGs are the major lipid species in S. aureus N315. The abundances of PGs 30:0 to 36:0 (C), lysyl-PGs 30:0 to 36:0 (D), CLs 56:0 to 63:0 (excluding 59:0) (E), PAs 30:0 to 36:0 in N315 and N315-D8 (F), and DGDGs 30:0 to 36:0 (G) are shown. (H) The abundances of FFAs that were differentially abundant in N315 and N315-D8 are also shown. Data shown in panels C to H are normalized to dry pellet weight, and error bars represent the standard deviation of the mean. The significance of differences was determined by Student's t test. *, P ≤ 0.05; **, P ≤ 0.005.