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. 2017 Dec 13;2(6):e00492-17. doi: 10.1128/mSphere.00492-17

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Copyright © 2017 Hines et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Results of analyses of daptomycin-susceptible (W40308) and -resistant (W49297) C. striatum by HILIC-IM-MS. (A) Positive-mode IM-XIC of W40308 (raw intensity [Int.] of 183-mg dry pellet shown) reveals that AlaPGs, PGs, and PIs are the major lipid species in daptomycin-susceptible C. striatum. (B) The major lipid species in daptomycin-resistant C. striatum strain W49297 (raw intensity of 41-mg dry pellet shown) are GlcADGs and PIs in positive mode. (C) The abundances of the major lipid species in C. striatum observed in the negative-mode (−ESI) HILIC-IM-MS analysis. (D) The abundances of the major lipid species observed in the positive-mode (+ESI) HILIC-IM-MS analysis. Data shown in panel C and D are normalized to dry pellet weight, and error bars represent the standard deviation of the mean. Significance was determined by Student's t test. **, P ≤ 0.005.