FIG 1 .

Human antibody response to vaccination with recombinant H7 HA as measured by HI assay (A and B) and ELISA (C and D). (A) HI titers of enrolled subjects (n = 382) at time points day 0 (D0), day 21, and day 42 postprime for the four different treatment groups. The dashed line represents an HI titer of 1:40, which was defined as seroconversion (4-fold increase in HI titer or HI titer of ≥1:40). The bars indicate the geometric mean (GM) of all data points. (B) Induction of HI titers over baseline after one vaccination (D21) and two vaccinations (D42). (C) Absolute ELISA AUC values of antibodies binding to matched HA of A/Anhui/1/2013 after vaccination with recombinant H7 HA. (D) Induction for the time points day 21 and day 42 postvaccination for the four different treatment groups. The results are presented as GM values relative to baseline. In panels A and C, time points day 0, day 21, and day 42 were compared to each other within a treatment group in a one-way ANOVA. In panels B and D, each day 21 time point was compared to every day 21 time point of all other treatment groups. The same comparison was performed for the day 42 time point. Significance is indicated as follows: no symbol, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. adj., adjuvant.