FIG 4 .

In vitro functionality of human serum antibodies induced by H7 vaccination. (A) ADCC AUC values measured for the four different treatment groups and the HI seroconverter group. The bars show the geometric means of the AUC values. (B) GMs of induction over baseline at day 42 after two vaccinations based on AUC values are represented for the HI seroconverter group (1.9-fold, green hexagons), 7.5-µg-plus-adjuvant group (2.8-fold, purple circles), 15-µg-plus-adjuvant (adj.) group (1.4-fold, yellow triangles), 30-µg-plus-adjuvant group (1.8-fold, blue squares), and 30-µg no-adjuvant group (1.2-fold, red diamonds). The dashed line represents a 1-fold increase in ADCC activity. (C) Individual titers of neutralizing serum antibodies in a microneutralization assay using the H7N9 A/Shanghai/1/2013 strain. The HI seroconverter group (green hexagons), high-dose (30-µg) adjuvanted group (blue squares), and 30-µg nonadjuvanted group (red diamonds) were selected for analysis. The dashed line represents the limit of detection (titer of 1:10), and the bars show the geometric means. (D) Increase in microneutralization (MN) titers over baseline at day 42. The dashed line represents a 1-fold increase in microneutralization titers. In panels A and C, time points day 0 and day 42 within one group were analyzed for significance values in a one-way ANOVA. In panels B and D, each column was compared to every other column. Significance is indicated as follows: no symbol, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.