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. 2017 Dec 13;13:386. doi: 10.1186/s12917-017-1284-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Validating the designed primers/probe of IBRV LFD-RPA assay. a Agarose gel electrophoresis of RPA products generated using designed primers/probes. Lane M: molecular weight standard (DNA Marker1000). Lane 1 to 9: designed primer and probe sets. Especially, Lane 5 was primers/probe set 4-2F/4-2R/4-2LF, and the expected size of the product was 247 bp. b Lateral-flow strip end-point analysis of RPA products generated using optimal designed primers/probes set 4F/4R/4LF. Lane 1: positive control (supplied by Twist Ampnfo kit); Lane 2: negative control (DNase-free water); Lane 3: IBRV amplicons performed with RPA primer pair 4F/4R/4LF. Samples were tested in triplicate with one reaction displayed in figure for each triplicate