Table 1.

The sequences of primers and probes designed in this study

Primers	Oligonucleotide sequences (5’to3’)	Product sizes (bp)	Location
1F:	5′-GGCAAGCGCCGCGAAGGAAACCAAGTCG-3′	236	4013–7237
1R:	【Biotin】 5′-GGCTTTACTCCAACAGCGGTGCGACTCCCTTC-3’
1LF:	5′-【FAM】GCGAAGGAAACCAAGTCGGGGAAGGCGAGTC[dSpacer]CGAGGGGTTAGGCG【C3-spacer】-3’
2F	5′- GGGGACGGCCACGTACACTTGCTCGTCGTAGAGG −3′	187	19,597–20,823
2R:	【Biotin】5′- TCTGTCCGACGGCGGCAACGGCAACCCAATC −3’
2-1LF1:	AAAGGCGTTTCGCAGCGAGGCGCCAAAGGG[dSpacer]CTCAGCAGGGCGGAGAC-3′
2-2LF2:	5′-【FAM】AGGCGTTTCGCAGCGAGGCGCCAAAGGGG[dSpacer]TCAGCAGGGCGGAGACGT【C3-spacer】-3’
3F:	5′- ACACGAGGTCGCCGCCCGTGAAGACGCCGTTGTT −3′	142	23,526–25,889
3R	【Biotin】5′- CCAATGGACGGAAAGGTGCCCGCCTCCCAGAT −3’
3LF:	5′-【FAM】CCGTTGTTGGTGGCCTTGCGGATCTTGCAG[dSpacer]AGTAGAGCCCCGTCTTA【C3-spacer】-3’
4-1F:	5′- TTGACAAAGACCGAGTCCGTGTCGCCGTAAACCA −3′	274	45,238–48,978
4-1R:	【Biotin】5′- TCGATAAGCAGCAGGCCGCCATCAAGGTCGTGT −3′
4-1LF:	5′-【FAM】TGTGGCCCAGCGCTCGTGTATGTACCGCCG[dSpacer]GTCTCGAGCAGCATGTC【C3-spacer】-3’
4-2F	5′-GCTTTCCGACCAGCGCCGAATCTTGGATGCAGT −3′	250	47,151–47,397
4-2R:	【Biotin】5′- GGCCAAAACCGCTTTCAGAAGCAGAGCAAGGTGA −3’
4-2LF:	5′-【FAM】CCGCAAAGTGCCGAGGGATGTCCTTGTAGT[dSpacer]CAGGTCCACCTTCCGCT【C3-spacer】-3’
4-3F:	5′- GCCACCGCCGACAGCTCCAGGTGGGGCAAGTAC −3′	295	47,151–47,397
4-3R:	【Biotin】5′- CGGGCCAAAACCGCTTTCAGAAGCAGAGCAAGGT −3’
4-3LF:	5′-【FAM】CACCTTCCGCTCCCGCAGGGCCTCCTCGGC[dSpacer]ACGGCGTTGAGCTTGTA【C3-spacer】-3′
4-4F	:5′- CTTTCCGACCAGCGCCGAATCTTGGATGCAGTA −3′	154	47,151–47,397
4-4R:	【Biotin】5′- CAGCTACAAGCTCAACGCCGTGGCCGAGGAGGC −3’
4-4LF:	5′-【FAM】CCGCAAAGTGCCGAGGGATGTCCTTGTAGT[dSpacer]CAGGTCCACCTTCCGCTC【C3-spacer】-3′
gB-F:	5′- ACACGGACCGCGTGCCCGTGGGCATGGGCGAGATCA −3′	257	55,015–58,579
gB-R:	【Biotin】5′- GCAGTTCACAGAGGTGCCCGTGCGGTAGAGCC −3’
gB-LF:	5′-【FAM】AGCGGGCGCAAGGTGGTGGCCTTTGACCGC[dSpacer]ACGACGACCCCTGGGAG【C3-spacer】-3′

Note: F: Forward primer; R: Reverse primer; LF Probe; FAM Carboxyfluorescein; dSpacer A tetrahydrofuran residue; C3-spacer: 3′-block