Table 2.

RPA primers and probe used for the rapid detection of IBRV

Name	Sequence (5’to 3′)
4F	5′-TTCCGACCAGCGCCGAATCTTGGATGCAGTACT-3’
4R	5′-【Biotin】GGCCAAAACCGCTTTCAGAAGCAGAGCAAGGTGA-3’
4LF	5′-【FAM】CCGCAAAGTGCCGAGGGATGTCCTTGTAGT【dSpacer】CAGGTCCACCTTCCGCT【C3-spacer】-3’

Note: F Forward primer; R Reverse primer; LF probe; FAM Carboxyfluorescein; dSpacer A tetrahydrofuran residue; C3-spacer: 3′-block