Table 3.
Comparative performance of LFD-RPA, PCR and SYBR Green I real time qPCR assays for detection of IBRV suspected clinical specimens
| Samples name | PCR | LFD-RPA | Real-time qPCR | LFD-RPA/Real-time qPCR | LFD-RPA/ PCR/ Real-time qPCR | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| P | N | P | N | P | N | P | N | P | N | |
| fecal | 18 | 6 | 20 | 4 | 20 | 4 | 20 | 4 | 18 | 4 |
| blood | 30 | 6 | 32 | 4 | 32 | 4 | 32 | 4 | 30 | 4 |
| nasal swab | 20 | 18 | 24 | 14 | 24 | 14 | 24 | 14 | 20 | 14 |
| lymph node | 3 | 0 | 3 | 0 | 3 | 0 | 3 | 0 | 3 | 0 |
| spleen | 3 | 0 | 3 | 0 | 3 | 0 | 3 | 0 | 3 | 0 |
| lung | 2 | 0 | 2 | 0 | 2 | 0 | 2 | 0 | 2 | 0 |
| Total | 76 | 30 | 84 | 22 | 84 | 22 | 84 | 22 | 76 | 22 |
Note: P Positive; N Negative