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. 2017 Dec 13;16:242. doi: 10.1186/s12944-017-0629-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Two-channel fluorescence images of THP-1 derived macrophages exposed to various conditions. Nuclei were stained with DAPI (blue), lipids were stained with Nile Red (red), and cells were imaged at 20× magnification. Scale bar is 20 μm. -ApoB (pre-cultured with rabbit anti-apolipoprotein B antibodies), Chol (cholesterol and 1-palmitoyl-lyso-phosphatidylcholine (LPC) in PBS), CHS (cholesteryl hemisuccinate and LPC in PBS), Erg (ergosterol and LPC in PBS), LDL (low-density lipoprotein in PBS), LPS (lipopolysaccharide in PBS), PBS (phosphate-buffered saline vehicle), Ser (medium contained 10% fetal bovine serum)