Figure 2.

Xenotransplantation of MDS BM CD34⁺ cells. (a) Percentage of hCD45⁺ engraftment observed in NSG mice injected with CD34⁺ cells from various MDS WHO subtypes (patients n=22; NSG mice n=74). hCD45 ⩾0.01% in total mouse BM was considered as successful human cell engraftment. (b) hCD45⁺ engraftment in total mouse BM at the time of killing from NSG mice injected with patient CD34⁺±MSCs (autologous/allogeneic) (CD34⁺ alone n=35 mice and patients n=22, CD34⁺+nMSCs n=6 mice and patients n=6; CD34⁺+pMSCs n=12 mice and patients n=6). nMSCs, normal healthy donor MSCs, pMSCs, autologous patient MSCs. (c) Comparison of hCD45⁺ engraftment observed in injected bone vs noninjected bones from NSG mice transplanted with patient CD34⁺+MSCs (mice n=10, patients n=6 (MDS 6, 9, 17, 18, 20 and 27).