Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 2;31(12):2543–2551. doi: 10.1038/leu.2017.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

WT1 interacts with TET2 to co-regulate MEG3 expression in AML cells. (a) RT-qPCR analysis of MEG3 RNA expression after transfected with four major WT1 splicing variants (+5/+KTS, +5/−KTS, −5/+KTS, −5/−KTS) in U937 and NB4 cell lines. (b) Induction of MEG3 promoter activity by WT1 in U937 and NB4 cell lines. (c) The WT1 binding at the promoter regions of MEG3 was assessed by ChIP analysis. (d) HL-60 cell line was transfected with a Flag-TET2^CD construct, western blotting analysis of TET2 and Flag, RT-qPCR analysis of MEG3 RNA expression. (e) A representative methylation pattern of the CpGs in K562 cells after bisulfite treatment. Each line represented one PCR product, and six PCR products were shown for each sample. (f) Induction of MEG3 promoter activity by TET2^CD or TET2^CM in U937 cells. (g) WT1 was transiently overexpressed either singly or with shRNA against TET2 in U937 and NB4 cells, RT-qPCR was examined for the RNA expression of MEG3. (h) Induction of MEG3 promoter activity by WT1 either singly or with shRNA against TET2 in U937 and NB4 cell lines. (i) WT1 was transiently overexpressed either singly or shRNA against TET2 in U937 and HL-60 cells. The WT1 binding at the promoter regions of MEG3 was assessed by ChIP analysis. Representative images of three independent experiments were shown. ACTB protein was used as an internal control for western blotting analysis.*P<0.05; **P<0.01. •, methylated CpG; ○, unmethylated CpG; ChIP, chromatin immunoprecipitation; NS, not significant; RT-qPCR, real-time quantitative PCR; shRNA, short hairpin RNA.