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. 2017 Aug 29;41(12):1805–1814. doi: 10.1038/ijo.2017.179

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Copyright © 2017 The Author(s)

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Gr1^low monocytes are increased in DIO, and the integrin CD11c is highly upregulated on Gr1^low monocytes during HFD in C57BL/6 mice. (a) The frequency of monocytes was determined by flow cytometry in C57BL/6 female mice fed a chow diet (white histograms) or a HFD containing 60% kcal (black histograms) from fat for 30 weeks, based on expression of CD45, CD11b and CD115 (respectively, n=6 and 12). (b, c) Relative frequencies of Gr1^low and Gr1^high subsets, in percentage of leucocytes (CD45⁺) (b) or of monocytes (c). (d) Ratio of Gr1^low/Gr1^high monocytes. (e, f) Dot plots of the CD11c expression on Gr1^low and Gr1^high monocytes (e: chow diet; f: HFD). *P<0.05. **P<0.01. Data are expressed as means±s.d.