Figure 2.

Gr1^low monocytes are decreased in CX3CR1^−/− HFD-fed mice, and CX3CR1 deficiency results in decreased BW gain but increased IR following exposure to HFD. (a) The frequency of monocytes was determined in C57BL/6 or CX3CR1^−/− female mice fed a chow diet (white histograms) or a HFD containing 60% kcal from fat (black histograms) for 30 weeks, as in Figure 1 (respectively, n=9 and 12). (b) Relative frequencies of Gr1^low and Gr1^high monocytes, in percentage of leucocytes (CD45⁺). (c) Ratio of Gr1^low/Gr1^high monocytes. (d) Weight gain in CX3CR1^−/− mice and control. Mice were fed a diet of 40% kcal from fat until 18 weeks then shifted to a diet of 60% kcal from fat (n=6 controls and 10 CX3CR1^−/− mice). (e) AT weights in CX3CR1^−/− and control mice fed 30 weeks with the HFD described in e. (f, g) Plasma glucose during GTT (f) and ITT (g) after 12 h fasting after 28 weeks of HFD for GTT and 30 weeks of HFD for ITT (n=5/4; HFD containing 40% followed by 60% kcal from fat). *P<0.05. **P<0.01. ***P<0.001. Data are expressed as means±s.d.