Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 29;41(12):1805–1814. doi: 10.1038/ijo.2017.179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Characterization of AT macrophage in CX3CR1^−/− fed a HFD. (a) Dot plots of macrophages (F4/80⁺CD11b⁺CD45⁺ cells) in AT in CX3CR1^−/− mice and control mice fed a diet of 60% kcal from fat for 30 weeks. (b) Histograms represent ATM content assessed by flow cytometry in CX3CR1^−/− mice (n=9) and WT controls (n=8) treated as in a. (c) Characterization of M2 and M1 macrophages based on the expression of CD11c and Mgl1. Characterization of double-positive and double-negative macrophages for the two markers CD11c and Mgl1. Ratio of M2/M1 macrophages in mice described in b. (d) Levels of mRNA expression of functional M1 and M2 markers were evaluated by qPCR in adipose tissue from mice described in a (n=4 controls and 4 CX3CR1^−/− mice, representative of two independent experiments). Relative mRNA expression of M1 and M2 genes was normalized to the average expression of two housekeeping genes (hypoxanthine guanine phosphoribosyl transferase and ribosomal protein S3). *P<0.05. Data are expressed as means±s.d.