Figure 5.

Bcl-2 expression in CD11c⁺ cells increases the Gr1^low monocyte proportion, insulin sensitivity and M2 ATM content in DIO conditions. (a) Blood monocyte levels characterized as in Figure 1 in irradiated C57Bl/6 mice recipients of WT (white histograms, n=8) or transgenic CD11c-hBcl2 BM cells (gray histograms, n=9) fed a HFD for 22 weeks. (b) Relative frequencies of Gr1^low and Gr1^high monocytes in mice described in a. (c) Histogram shows the ratio Gr1^low/Gr1^high monocytes in mice described in a–c. (d) Weight gain in irradiated C57Bl/6 mice transferred with Tg CD11c-hBcl2 BM cells (gray) or with WT BM cells (white). Mice fed a diet of 60% kcal from fat (n=10 Tg CD11c-hBcl2 and 10 controls). Plasma glucose during GTT (e) and ITT (f) in mice described in d fed a HFD for 17 weeks and after 12 h fasting (n=6/6). (g) ATM content in mice described in a. Characterization of ATMs based on the expression of Mgl1 and CD11c. Ratio of M2/M1 macrophages. (h) Levels of mRNA expression of functional M1 and M2 markers were evaluated by quantitative PCR in AT from mice described in a (n=10 controls and 7 CD11c-hBcl2 transgenic mice). Relative mRNA expression of M1 and M2 genes was normalized to the average expression of two housekeeping genes (hypoxanthine guanine phosphoribosyl transferase and ribosomal protein S3). *P<0.05. **P<0.01. Data are expressed as means±s.d.