Fig. 3.

Analysis of MMP-9 production by airway epithelial cells exposed to poly(I:C) and TGF-β. a MMP-9 production was investigated in submerged cultures by qPCR or ELISA dosage (n = 4). b Results of expression were then confirmed in ALI culture conditions by ELISA (n = 6). c Use of TLR3/dsRNA complex inhibitor (614310) shows the role of TLR3 in MMP-9 production (n = 3). d Primary human AEC were cultured for 24 h with increasing doses of TGF-β, and MMP-9 production was measured by qPCR or ELISA dosage (n = 3). Fibronectin expression was investigated by qPCR (n = 3) or western Blotting. e qPCR analysis of BAMBI expression in submerged or ALI cultures exposed to TGF-β and/or poly(I:C) for 24 h (n = 3). Number of independent experiments is mentioned for each panel. Statistical significances were determined with a one-way ANOVA followed by a Tukey’s post-hoc test