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The overall scheme of the MS-based pipeline for the development of novel pancreatic cancer biomarkers. The pipeline utilised a serial of MS-based methods such as iTRAQ-2DLC-MS/MS Strategy, 1D-targeted LC-MS/MS, P-MRM and SID MRM to construct a coherent, high-throughput and non-discriminatory pipeline with strict quantity control. The performance of newly developed biomarkers was statistically evaluated by ROC curves. ROC = receiver operation characteristic.
