Table 1.
Sequences of oligonucleotide primers used for qPCR analysis of lung tissue
| Gene IDa | Primers (5′-3′) | Accession No. | Amplicon length |
|---|---|---|---|
| MUC5AC | Forward: CAGTACAGAGTGCATGGGGA Reverse: TTCACAAACACCTCCCCACT |
XM_015470102.1 | 185 |
| MUC5B | Forward: AAAACGCCCTTCACCTTCAC Reverse: TGCCTCAGGTTCTCGAATGT |
XM_015470101.1 | 176 |
| LAP | Forward: CCCTGGAAGCATGAGACAGA Reverse: TTTCTGACTCCGCATCCAGT |
S76279.1 | 109 |
| SP-A | Forward: TGGGGAGGCATCTTGTTAGG Reverse: TGTTCATCAGCAGGCAGGTA |
NM_001077838.2 | 194 |
| SP-B | Forward: GACATGTGGAAGCCGATGAC Reverse: TGAGTCCTGGAAAATGGCCT |
NM_001075311.2 | 92 |
| SP-C | Forward: GAGATCCAGGAGCAAAGGGT Reverse: CCTCCCACAGTCCCATTTCT |
NM_174462.4 | 95 |
| SP-D | Forward: CCTGTACCCTGGTCATGTGT Reverse: AGCAGAGCCATTGTCTCCTT |
NM_181026.2 | 179 |
| CYP1A1 | Forward: GTCACAACTGCCCTTTCCTG Reverse: AAAGGAGGAGTGTCGGAAGG |
XM_005192890.3 | 180 |
| CYP1A2 | Forward: TCCTCTTCCTGGCCATCTTG Reverse: CAGAACGCCAGCAACTTCTT |
XM_015468527.1 | 165 |
| ABCA3 | Forward: GAGCACACCTTCAACCACAG Reverse: AAAGGAGCCTGTCTGAGTCC |
NM_001113746.1 | 108 |
| SCN11A | Forward: ATGCTGGCTTTAATCTGCGG Reverse: CCTGGAAGCACGAATGGATG |
NM_174598.3 | 177 |
| SCN11B | Forward: CTGAAGGACCTGGACGAACT Reverse: TTGATAAAGACCAGGGGCGT |
NM_001098075.1 | 128 |
| SGK-1 | Forward: GGCTCGATTCTATGCTGCTG Reverse: ACGTTGTGCCATTGTGTTCA |
NM_001102033.1 | 121 |
a MUC5AC mucin 5 ac, MUC5B mucin 5B, LAP lingual antimicrobial peptide, SP-A Surfactant protein A, SP-B Surfactant protein B, SP-C surfactant protein C, SP-D surfactant protein D, CYP1A1 cytochrome P45 family 1 subfamily A member 1, CYP1A2 cytochrome P450 family 1 subfamily A member 2, ABCA3 ATP binding cassette subfamily A member 3, SCN11A Sodium channel voltage-gated type 2 alpha subunit, SCN11B Sodium channel voltage-gated type 2 beta subunit, SGK-1 Serum/glucocorticoid regulated kinase 1