Table 2.
Sequences of oligonucleotide primers used for qPCR analysis of jejunum tissue
| Gene IDa | Primers (5′-3′) | Accession No. | bp2 |
|---|---|---|---|
| NR3C1 | Forward: TACAGGCAGCAATGGTCTCA Reverse: AAGAGGGTGGTCATTCTGGG |
NM_001206634.1 | 154 |
| MUC1 | Forward: CCCAACTCTGTTCTGGGCTA Reverse: TTCCAGCCAGTATTCCAGCA |
AJ400824.1 | 163 |
| MUC2 | Forward: TGCTACTACGTGCTGACCAA Reverse: ACGTTCTTCTTGTTGTCGGC |
XM_010806231.2 | 131 |
| TNF- α | Forward: CGTGGACTTCAACTCTCCCT Reverse: GGACACCTTGACCTCCTGAA |
NM_173966.3 | 179 |
| Il-1 β | Forward: CCAGCTGCAGATTTCTCACC Reverse: TCACACAGAAACTCGTCGGA |
NM_174093.1 | 195 |
| il-4 | Forward: TGCCCCAAAGAACACAACTG Reverse: GAACAGGTCTTGCTTGCCAA |
NM_173921.2 | 144 |
| Il-6 | Forward: ACTTCTGCTTTCCCTACCCC Reverse: TGTCGACCATGCGCTTAATG |
NM_173923.2 | 121 |
| VIP | Forward: TCGACTCCCAGGACTTCAAC Reverse: GAGAAGAGCACGCTGAACAG |
NM_173970.3 | 148 |
| FCGRT | Forward: ACTATCGCTCGCTCCAGTAC Reverse: CCTGCGCCCGTAGATTATTG |
NM_176657.1 | 126 |
| B2M | Forward: GTTCACTCCCAACAGCAAGG Reverse: TCTCGATGGTGCTGCTTACA |
NM_173893.3 | 109 |
| RAB11A | Forward: GCAACAAGAAGCATCCAGGT Reverse: TAAGGCACCTACAGCTCCAC |
NM_001038162.2 | 120 |
| RAB25 | Forward: AGCTGAGAGTTGAGGGCATT Reverse: TCGGCTCTGTTTCCCATCTT |
NM_001017936.1 | 102 |
| STX3 | Forward: GAGCAGCATCAAGGAGCTTC Reverse: TACCAATTTCTTCCGGGCCT |
NM_001101971.1 | 181 |
| pIg | Forward: GGTGTGCTGGTTCCTTCTTG Reverse: ATAAGCTGCAGGTGGGAACT |
NM_174143.1 | 93 |
| MYO5B | Forward: GAAGCAATACCGCATGCAGA Reverse: TTCTGGATGATGGTGGCCTT |
XM_015469176.1 | 147 |
a NR3C1 Nuclear receptor subfamily 3 group C member 1, MUC1 Mucin 1, MUC2 Mucin 2, TNFα Tumor necrosis factor alpha, IL-1β Interleukin 1 beta, IL-4 Interleukin 4, IL-6 Interleukin 6, VIP Vasoactive intestinal polypeptide, FCGRT fc fragment of Ig receptor and transporter, B2M Beta −2- microglobulin, RAB11A Ras related protein Rab11a, RAB25 Ras related protein RAB25, STX3 Syntaxin 3, pIg Polymeric immunoglobulin receptor, MYO5B Myosin VB