Table 4.
Sequences of oligonucleotide primers selected as reference genes for qPCR analysis
| Gene IDa | Primers (5′-3′) | Accession No. | Amplicon length |
|---|---|---|---|
| Act- β | Forward: CGGCATCGAGGACAGGAT Reverse: CATCGTACTCCTGCTTGCTGAT |
NM_173979.3 | 169 |
| GAPDH | Forward: CCTGCCCGTTCGACAGATA Reverse: GGCGACGATGTCCACTTTG |
NM_001034034.1 | 150 |
| FAU | Forward: CCGCATGCTTGGAGGTAAAG Reverse: CACAACATTGACAAAGCGCC |
NM_174731.3 | 154 |
| RPS9 | Forward: CCGCATGCTTGGAGGTAAAG Reverse: CACAACATTGACAAAGCGCC |
NM_001101152.2 | 188 |
| HPRT1 | Forward:GGATTACATCAAAGCACTGAACA Reverse: CATTGTCTTCCCAGTGTCAATT |
NM_001034035 | 194 |
a Act- β β-actin, GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase, FAU Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed, RPS9 Ribosomal Protein S9, HPRT1 Hypoxanthine Phosphoribosyltransferase 1