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. 2017 Oct 20;25(1):217–225. doi: 10.1038/cdd.2017.168

Figure 1.

Figure 1

BIM-deficient cells have increased mitochondrial oxygen consumption rate (OCR) and reduced mitochondrial potential. (a) Mitochondrial OCR was measured in wild-type, BIM−/− and BAX−/−BAK−/− MEFs using a Seahorse XF24 bioanalyzer, n=3 independent MEF isolations/genotype. After recording three baseline OCR measurements, oligomycin (Oligo), FCCP, rotenone (Rot) and antimycin A (Anti) were sequentially added to the cells and OCR measurements taken thrice after each treatment. (b) Quantification of parameters of mitochondrial respiration measured by the Seahorse bioanalyzer. n=3 independent experiments. ***P<0.001 BIM−/− versus wild-type MEF (one-way ANOVA). (c) Mitochondrial potential was measured in wild-type, BIM−/− and BAX−/−BAK−/− MEFs by JC-1 staining. n=3 independent experiments, *P<0.05 BIM−/− versus wild-type MEFs (one-way ANOVA). Data show mean±S.E.M.