Figure 2.
mRNA expression of cells collected from different fractions of enzymatic digestion of tooth. (a) BSP, DMP-1 and ALP expression. (b) IL-1 receptor 1 and receptor 2 expression. (c) RANKL and OPG expression after treatment with IL-1β (mixture: cells from 60–90 min fractions). ALP, alkaline phosphatase; BSP, bone sialoprotein; DMP-1, dentin matrix acidic phosphoprotein 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HCEM, human cementoblast cell line; hPDL, primary human periodontal ligament cells; IL, interleukin; OPG, osteoprotegerin; pHCB, primary human cementoblasts; RANKL, receptor activator of nuclear factor kappa-B ligand.