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. 2017 Dec 13;9(12):e5. doi: 10.1038/ijos.2017.45

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© The Author(s) 2017

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Co-culture of cementoblasts and primary human peripheral blood monocytes for 21 days. (a) TRAcP and nuclear staining, scale bar 100 μm. (b) Osteoclast number and the number of cells including different nuclei number. (c) The average number (black dots) of nuclei per generated osteoclasts (independent samples comparison t-test, n=5). HCEM, human cementoblast cell line; hPBMC, human peripheral blood monocyte; IL, interleukin; pHCB, primary human cementoblasts; TRAcP, tartrate-resistant acid phosphatase.