Skip to main content
. Author manuscript; available in PMC: 2017 Dec 14.
Published in final edited form as: Leuk Res. 2016 Sep 26;50:85–94. doi: 10.1016/j.leukres.2016.09.018

Table 2.

The sequences of primers and conditions for bisulfite-sequencing PCR.

Name Analyzed regions relative to transcriptional start site of NR4A3 No. of analyzed CpGs Forward (5′ → 3′) Reverse (5′ → 3′) Annealing temperature (°C) Cycle Length(bp)
NR4A3-Region 1 −551 to −24 51 TTATGTAAGAGGAAAGTGTAGTT CTCCATAAAATACCTAAAATAC 54 48 528
NR4A3-Region 2 −37 to +485 47 AGGTATTTTATGGAGAG AAAAATCACAACTACTATCC 54.4 40 522
NR4A3-Region 3 +6151 to +6535 15 TTTGTTTTTGAGAGATTTTTTTT AATAACTACTAATACTACTAC 53.2 45 385
NR4A3-Region 4 +7116 to +7432 9 GAGGGTTGTAAGGGTTTTTT CAAATCCTTTTAAAACTCTAC 57.7 40 317