Figure 4.

Myeloid and lymphoid chimerism in humanized CA mice after BMT in the absence of cytoreductive conditioning. (A) Bone marrow cells from CA mice 20 weeks after transplantation were stained with fluorescently labeled antibodies that recognize myeloid (Gr-1 and Mac1) and lymphoid (B220) antigens and analyzed by flow cytometry. Donor-derived myeloid and lymphoid cells were determined by GFP fluorescence. Chimerism levels are determined by the ratio of GFP-positive cells to total positive cells for each lineage antibody tested. For this representative mouse, donor-derived Gr-1, Mac1, and B220-positive cells were 7.8% (0.54/6.91), 8.0% (1.13/14.2), and 9.1% (4.88/53.7), respectively. (B) Donor chimerism was consistent between multiple lineages and HSPCs. Overall, the mean (± SEM) granulocyte (Gr-1), macrophage (Mac1), B-cell (B220), and HSPC (LSK) donor chimerism levels for 6 of the transplanted CA mice tested were 5.2% (±1.6%), 4.5% (±1.3%), 4.2% (±1.4%), and 7.4% (±2.1%), respectively.