FIG. 3.

Overexpression of Prmt6 increased H3R2 methylation at promoters of pluripotency genes. Chromatin immunoprecipitation (ChIP) was performed using (A) anti-Prmt6 antibody, (B) anti-H3R2me2 antibody, and (C) anti-H3K4me3 respectively. ChIP DNA was analyzed by quantitative real-time polymerase chain reaction (PCR) with primers located at Oct4 and Nanog promoter regions. Fold enrichments were calculated from the apparent IP efficiency (ratio of ChIP-enriched DNA over input) and normalized to the level at a control region defined as 1.0 for a given extract.