FIG. 4.

Overexpression of Prmt6 decreased H3K4 mono-methylation, Wdr5, and Chd1 at promoters of pluripotency genes. ChIP was performed using (A) anti-H3K4me2 antibody, (B) anti-H3K4me antibody, (C) anti-H3K9me3 antibody, (D) anti-Wdr5 antibody, and (E) anti-Chd1 antibody respectively. ChIP DNA was analyzed by quantitative real-time PCR with primers located at Oct4 and Nanog promoter regions. Fold enrichments were calculated from the apparent IP efficiency and normalized to the level at a control region defined as 1.0 for a given extract.