FIG. 5.

Prmt6 RNAi results in ES cell differentiation. (A) Depletion of Prmt6 increases the expression level of certain lineage markers, such as Fgf5. Mean levels are expressed relative to the vector control and normalized to beta-actin expression levels. (B) Immunoblot with chromatin extracts from GFP RNAi cells and Prmt6 RNAi ES cells using antibodies against Prmt6, histone H3 marks (H3K4me3 and H3R2me2), or pluripotency markers (Nanog and Oct4). Beta-actin and histone H3 served as loading controls. (C) Prmt6 RNAi ES cells partially lost the activity of undifferentiated ES cell marker, AP (scale bar=100 μm), compared to the control cells. Color images available online at www.liebertonline.com/scd