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. 2017 Dec 11;10:5873–5882. doi: 10.2147/OTT.S144280

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© 2017 Dong et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The effects of miR-149* and GIT1 on cell proliferation, invasion, and migration.

Notes: (A) The miR-149* and GIT1 expressions were measured by qRT-PCR to demonstrate the transfection efficiency of NC, miR-149*, miR-149* inhibitor, si-GIT1, and miR-149* + siGIT1 plasmids. (B) Cell proliferation was performed by MTT assay. **P<0.01. (C) A Matrigel transwell invasion assay was used to evaluate the invasive ability of MDA-MB-231 cells subjected to different plasmids’ transfection. Representative images of the invasion assays for MDA-MB-231 cells are displayed in the left panel, while statistical histogram is exhibited in the right panel. **P<0.01. (D) A transwell migration assay was applied to evaluate the migrative ability of MDA-MB-231 cells subjected to different plasmids’ transfection. Representative images of the invasion assays for MDA-MB-231 cells are displayed in the left panel, while statistical histogram is exhibited in the right panel. **P<0.01.

Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NC, negative control; OD, optical density; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.

The effects of miR-149* and GIT1 on cell proliferation, invasion, and migration.

Notes: (A) The miR-149* and GIT1 expressions were measured by qRT-PCR to demonstrate the transfection efficiency of NC, miR-149*, miR-149* inhibitor, si-GIT1, and miR-149* + siGIT1 plasmids. (B) Cell proliferation was performed by MTT assay. **P<0.01. (C) A Matrigel transwell invasion assay was used to evaluate the invasive ability of MDA-MB-231 cells subjected to different plasmids’ transfection. Representative images of the invasion assays for MDA-MB-231 cells are displayed in the left panel, while statistical histogram is exhibited in the right panel. **P<0.01. (D) A transwell migration assay was applied to evaluate the migrative ability of MDA-MB-231 cells subjected to different plasmids’ transfection. Representative images of the invasion assays for MDA-MB-231 cells are displayed in the left panel, while statistical histogram is exhibited in the right panel. **P<0.01.

Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NC, negative control; OD, optical density; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.