Figure 2.

NEAT1 knockdown inhibited proliferation and enhanced apoptosis in NPC cells. (A) The expression level of NEAT1 was detected by RT-qPCR in si-NC- or si-NEAT1-transfected NPC cells. (B) The effect of NEAT1 knockdown on the colony-forming capacity of SUNE2 and CNE1 cells was assessed by colony formation assay. (C) The viability of NPC cells in which si-NEAT1 was introduced was determined by Cell Counting Kit-8 at OD 450 nm. (D) Flow cytometry was employed to evaluate the effect of NEAT1 depletion on apoptosis in NPC cells following treatment with Annexin V-FITC Apoptosis Assay kit; *P<;0.05 vs si-NC.

Abbreviations: NPC, nasopharyngeal carcinoma; RT-qPCR, reverse transcription–quantitative polymerase chain reaction; NC, negative control; PI, propidium iodide.