Figure 3.

NEAT1 suppressed miR-124 expression by direct interaction. (A) Sequence alignment of miR-124 with the putative binding sites within NEAT1 and mutant miR-124 binding sites. (B) Dual-luciferase reporter assays were used to investigate whether NEAT1 could directly interact with miR-124 by the putative binding sites in SUNE2 cells cotransfected with wild-type (WT) or mutant-type (MUT) NEAT1 luciferase vectors and miR-NC, miR-124, anti-miR-NC, or anti-miR-124. (C) SUNE2 cells lysate was treated with anti-Ago2 or anti-IgG (negative control) for RNA immunoprecipitation (RIP) assay. NEAT1 expression (D) and miR-124 expression (E) in NEAT1- or si-NEAT1-transfected SUNE2 cells are presented; *P<;0.05 vs respective control.

Abbreviation: NC, negative control.