Figure 5.

MiR-124 inhibited cell proliferation and induced apoptosis by regulating NF-κB signal. MiR-NC- or miR-124-transfected SUNE2 cells were treated with or without 10 ng/mL TNF-α. (A) Relative activity of NF-κB was analyzed by TransAM NF-κB p65 kit in treated cells. (B) Western blot analysis was performed to measure phosphorylation of IκBα and p65 in treated cells. β-actin was used as the internal reference. Colony-forming ability (C), cell viability (D), and apoptosis (E) were analyzed in treated cells; *P<;0.05 vs respective control.

Abbreviations: NC, negative control; TNF-α, tumor necrosis factor-α.