Figure 6.

NEAT1 promoted tumor growth through inhibiting miR-124 expression in vivo. About 8×10⁶ SUNE2 cells stably transfected with lenti-control or lenti-NEAT1 were subcutaneously injected into the nude mice. One week later, intratumor injection of PBS, miR-NC, or miR-124 mimics were performed once a week for 6 consecutive weeks according to indicated groups (n=6 in each group): lenti-control+PBS, lenti-NEAT1+PBS, lenti-NEAT1+miR-NC, lenti-NEAT1+miR-124. Mice were euthanized for removing tumor masses at 7 weeks after inoculation. (A) The tumor volume was measured with a caliper once. (B) Representative images of the xenograft tumors isolated from different groups. (C) The average weight of resected tumors. RT-qPCR analysis was applied to test the expression of NEAT1 (D) and miR-124 (E) in excised tumor tissues; *P<;0.05 vs corresponding control.

Abbreviations: NC, negative control; RT-qPCR, reverse transcription–quantitative polymerase chain reaction.