FIG. 3.

One-week evaluation of cell proliferation resulting from in vivo FGF-2 delivery by the bCaP-PEM coated scaffold. (A) Schematic representation of the 3.5 mm critical-sized calvarial bone defect showing the histological sectioning and central defect region analyzed. (B) Fluorescence images of the entire calvarial defect showing DAPI stained (blue) and EdU-labeled (yellow) cells with white triangles indicating original bone defect edges. White box indicates area of higher magnification shown in (C). (C) Higher magnification images show increased EDU staining in scaffolds with FGF-2 and BMP-2 compared to BMP-2 alone. (D) Quantified results from Image J analysis of the histological sections shown in (C). Scale bar = 200 μm. BMP-2, bone morphogenetic protein-2; PEM, polyelectrolyte multilayer. Color images available online at www.liebertpub.com/tea