Extended Data Figure 2. Naïve T cells from Mettl3 KO mice differentiated into less Th1 and Th17 cells, more Th2 cells ex vivo comparing to WT naïve T cells.

a, Naïve T cells isolated from Mettl3 WT and KO mice were differentiated into effector subsets under defined optimal conditions. b, The percentages of each T cell subtypes over total CD4+ T cells were analyzed by FACS. c, No apoptosis defects were found in ex vivo cultured cells from WT and Mettl3 KO naïve T cells by FACS staining of Annexin V and 7AAD. Double negative stained cells are live cells, and the remaining are apoptotic cells. The percentage is listed in the right graph. d, No proliferation differences were found in ex vivo cultured cells from WT and Mettl3 KO naïve T cells. Naïve T cells labeled with CellTrace were culture ex vivo under different concentration of Anti-CD3/CD28 beads for 4 days. The percentages of proliferating cells were listed in the right graph.