Table 1. Summary of results from sequencing the HDTM library.
HDTM library preparation | Total reads | No. of reads containing the transposon sequence and mapping to the genome | G+C content of reads | No. of unique insertion sites | Frequency of Tn insertion |
---|---|---|---|---|---|
KAPA | 20 459 975 | 4 370 457 (21 %) | 61.0 % | 89 983 | 1/87 bp |
iTaq | 15 132 067 | 6 936 891 (46 %) | 59.7 % | 293 568 | 1/27 bp |