Lineweaver-Burk plot analysis of the inhibition mechanism of NAI. (A) Competitive
inhibition of oseltamivir at concentrations of 0.0, 0.1, 0.5 and 1 μM.
Regardless of the presence of oseltamivir, Vmax is invariant,
however, the increase in concentration of oseltamivir led to the raise of
Km. (B) Mixed inhibition of artocarpin at concentrations of 0, 5,
20 and 40 μM. The presence of artocarpin resulted in a decreased
Vmax and increased Km. The Ki values of
oseltamivir and artocarpin are 0.23±0.04 μM and 9.68±1.49
μM, respectively.