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. Author manuscript; available in PMC: 2017 Dec 14.
Published in final edited form as: New Phytol. 2017 Jun 28;215(3):1144–1155. doi: 10.1111/nph.14654

Fig. 2.

Fig. 2

Effects of AtRAP overexpression on disease resistance to Pseudomonas syringae pv. tomato (Pst) in Arabidopsis. (a) Phenotypes of the AtRAP knockout mutant and overexpression plants. (b) Immuno-detection of AtRAP-FLAG with anti-FLAG antibody in extracts from seven independent AtRAP overexpression lines. (c, d) Growth of Pst (EV) and Pst (avrRpt2) in two independent AtRAP overexpression lines at 0 and 3 dpi. Arabidopsis plants were infiltrated with Pst (EV) or Pst (avrRpt2) at a concentration of 2 ×105 CFU ml−1. At least six leaf disks were collected at 0 and 3 dpi by a cock borer. The 0 dpi samples were immediately collected after inoculation. Bacterial titers were measured by grinding, plating, culturing and counting colonies. An ANOVA test was used for significance difference calculation between Col-0 wild-type (WT) and overexpression lines. Error bars represent ±SD of more than six replicates. Similar results were obtained from three independent experiments. **, P < 0.01 (determined by Student’s t-test).