Fig 2. The expression of ydcFGH is driven by two promoters.
A. Schematic representation of the DNA fragments of the ydcFGH locus used for generating lacZ reporter fusions. The two putative promoters are indicated by arrows and the names of the resulting transcriptional fusion to lacZ are indicated below. On the right is displayed a picture of an X-Gal-LB plate to visualize LacZ activity of colonies harboring lacZ transcriptional fusions to Pydc1, Pydc2, Pydc1-2 or Pydc0 placed in either WT (ABS1761; ABS1763; ABS1765; ABS1767, respectively), or ΔydcH (ABS1820; ABS1821; ABS1822; ABS1823, respectively), and to Pydc1 or Pydc2 in ΔydcF (ASEC297; ASEC333) or ΔydcG (ASEC301; ASEC335) background. B. Expression of a Pydc1 luxABCDE transcriptional fusion in cells grown in LB medium, in a wild type (red; ABS2005) or mutant for ydcF (green; ASEC325), ydcG (purple; ASEC327) or ydcH (blue; ASEC329) background. Note that the ΔydcH data are relative to the upper part of the ordinate axis (in blue). Growth curves are presented as dotted lines and correspond to the optical density at 600nm while luciferase activities (plain lines) are relative luminescence units normalized by the OD600nm.