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. 2017 Dec 14;12(12):e0189694. doi: 10.1371/journal.pone.0189694

Fig 3. YdcH binds specifically to inverted repeats in the promoter region of ydcFGH.

Fig 3

A. Sequence of the region upstream of the ydcFGH operon. The two identified IR are indicated as green arrows. The transcriptional upshift previously identified is indicated as “up”, putative -35, -10 and rbs sequences are underlined, and the ydcF orf is boxed. B. EMSAs (right panels) showing the specific binding of PamR to DNA fragments corresponding to the wild type (wt) and mutated (IR1*) ydcF promoter, and schematic representation of the corresponding area (left panel). IRs are drawn as facing triangles, plain for the wild types and hollowed for the mutated. The quantity of YdcH (in pmol) incubated with 0.1 pmol of labeled target DNA is indicated above each lane.