Fig 4. Diet has little effect on glucose clearance and muscle physiology of Cpt1b^m-/- mice.

(A-B) GTT are shown for control (white circles) and Cpt1b^m-/- (black squares) mice that were fed (A) low fat diet (N = 8 animals per genotype) or (B) chow diet (N = 15 animals per genotype). (C) Levels in control (white) and Cpt1b^m-/- mice (black) of FGF21 mRNA in red quadriceps muscle (left panel), and FGF21 protein in serum; (right panel) (N = 8 animals per genotype for low fat diet and N = 10 animals per genotype for chow diet). (D) EM showing increased IMCL in soleus of Cpt1b^m-/- (right) mice relative to controls (left). (E-F) (N = 4 animals per genotype for low fat diet and N = 6 animals per genotype for chow diet) Relative levels of markers of mitochondrial biogenesis and lipid usage in control (white) and Cpt1b^m-/- (black) mouse red quadriceps muscle from mice fed (E) low fat diet or (F) chow diet. Asterisks indicate significance with P ≤ 0.05.